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Composite

Part:BBa_K515107:Design

Designed by: Atipat Patharagulpong   Group: iGEM11_Imperial_College_London   (2011-09-12)

Composite p(tetR) - Dendra2


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The original Dendra2 sequence had been joint codon optimized for Escherichia coli and Bacillus subtilis and designed to contain the insulator sequence under the influence of a strong RBS. However it could not be synthesised. We therefore allowed Eurofins to codon optimize it for E. coli and only had the coding region synthesised with a prefix and a suffix. Once the gene arrived, we assembled it using standard BioBrick assembly with the TetR repressible promoter (BBa_R0040) and a standard RBS (BBa_R0034).

Source

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References